MacGuidwin Nematology Lab, University of Wisconsin-Madison
We culture root lesion nematodes, Pratylenchus spp., in monoxenic culture using sweet corn and Gamborg’s B-5 medium. Each culture is descended from a single female nematode collected from an agricultural field in Wisconsin.
We culture root knot, Meloidogyne hapla, and soybean cyst, Heterodera glycines, on tomato or soybean, respectively, in soil. The cultures are descended from populations of nematodes collected from an agricultural field in Wisconsin.
Inoculum of Pratylenchus spp. consists of all vermiform stages and a small number of eggs in water. Inoculum of Meloidogyne hapla and Heterodera glycines consists of eggs in water. For all genera, the inoculum is shipped for next-day delivery in a single bottle with the concentration provided. .
All of the cultures are used for research in our lab so their infectivity and pathogenicity is evaluated regularly.
Root lesion nematodes are available year-round, although advance notice of an order is appreciated. Root knot and soybean cyst nematodes must be ordered 60 days in advance.
Please contact Ann MacGuidwin by email to place an order.
Fee Schedule for 2018:
|Pratylenchus penetrans (WI isolate)||$2.25 per 1000 nematodes|
|Pratylenchus spp.||$2.00 per 1000 nematodes|
|Meloidogyne hapla (WI isolate)||$2.50 per 1000 eggs|
|Heterodera glycines (WI isolate – specified.Hg type)||$1.75 per 1000 eggs|
|Heterodera glycines (WI isolate – specific Hg type*)||$1.50 per 1000 eggs|
|* more than 50 populations available representing a range of virulence phenotypes|
For more information please contact:
Dr. Ann MacGuidwin
Department of Plant Pathology
1630 Linden Drive
Madison, WI 53706